By Reinhard Neubert (Editor), Hans-Hermann Ruttinger (Editor)
This quantity provides breakthroughs and strategies in affinity capillary electrophoresis to degree and ascertain the physicochemical and thermodynamic parameters of drug compounds. It discusses techniques to discover and signify interactions to facilitate advancements in managed drug supply and focusing on.
Read Online or Download Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics (Drugs and the Pharmaceutical Sciences: a Series of Textbooks and Monographs) PDF
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Additional info for Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics (Drugs and the Pharmaceutical Sciences: a Series of Textbooks and Monographs)
Quantitative description of analyte migration behavior based on dynamic complexation in capillary electrophoresis with one or more additives. Electrophoresis 18:706–716, 1997. MT Bowser, AR Kranack, DDY Chen. Properties of multivariate binding isotherms in capillary electrophoresis. Anal Chem 70:1076–1084, 1998. S Terabe, K Otsuka, K Ichikawa, A Tsuchiya, T Ando. Electrokinetic separations with micellar solutions and open-tubular capillaries. Anal Chem 56:111– 113, 1984. KL Rundlett, DW Armstrong.
The shapes of the binding isotherms conﬁrm the stoichimetries of the complexes. Theory of Afﬁnity Electrophoresis Fig. 4 Calculated mobility with constant [I] at different concentrations of L. Fig. 5 Calculated mobility with constant [L] at different concentrations of I. 35 36 D. Ru¨ttinger Micellar Electrochromatography With micelles, microemulsions, or liposomes, a second phase is introduced into the separating system. As in chromatography, exchange of the analyte between the mobile and the stationary phases controls the separation process.
20) into the expression of the efﬁciency [Eq. (13)] and rearrangement yields an equation for the maximum number of theoretical plates per unit length: Nmax 1 u Ion = = l HETP 2D ͱ 4Wmax 1 и и d (21) From this equation it can be seen that the efﬁciency per unit length is inversely proportional to the capillary diameter. Decreasing the diameter of the capillary will decrease the height equivalent to a theoretical plate. The efﬁciency per unit length increases. Therefore, smaller-diameter capillaries can be used at shorter lengths, which ultimately decreases the separation time.
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