By Albert Yen Chang (auth.), Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki (eds.)
Animal mobilephone know-how is a transforming into self-discipline of mobilephone biology which goals not just to appreciate constructions, features and behaviors of differentiated animal cells but additionally to envision their skills for use in commercial and scientific reasons. The objective of animal telephone know-how contains accomplishments of clonal enlargement of differentiated cells with invaluable skill, optimization in their tradition stipulations, modulation in their skill to supply medically and pharmaceutically, vital proteins, and the applying of animal cells to gene remedy and synthetic organs. This quantity offers the readers an entire overview of current cutting-edge in Japan. The court cases could be helpful for phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines with regards to animal mobilephone tradition, operating in both educational environments or in biotechnology and pharmaceutical industries.
Read Online or Download Animal Cell Technology: Challenges for the 21st Century: Proceedings of the joint international meeting of the Japanese Association for Animal Cell Technology (JAACT) and the European Society for Animal Cell Technology (ESACT) 1998, Kyoto, Japan PDF
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Additional resources for Animal Cell Technology: Challenges for the 21st Century: Proceedings of the joint international meeting of the Japanese Association for Animal Cell Technology (JAACT) and the European Society for Animal Cell Technology (ESACT) 1998, Kyoto, Japan
Biotechnol. : High viable cell concentration fed-batch culture of hybridoma cells through on-line nutrient feeding. Biotechnol. Bioeng. 46 ( 1995) 579-587 EFFECTS OF SERUM AND OTHER MEDIUM COMPONENTS ON SUSPENSION CULTURE OF CHO CELLS PRODUCING TISSUE PLASMINOGEN ACTIVATOR M. C. H. JANG and T. YOSHIDA International Center for Biotechnology, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565, Japan Effects of the concentrations of serum and other medium components on cell metabolism in a suspension culture, in a spinner bottle, of CHO 1-15500 cells (ATCC CRL-9606) producing tissue plasminogen activator(tPA) were investigated in order to formulate an appropriate medium composition for perfusion culture.
HU, Z. ZHANG & M. uk/ChemEng/actg/ac tghomehtm ) Keywords: Cell attatchment, CHO cells, Porous microcarriers 1. Abstract CHO cells were successfully cultured on a novel type of microcarriers (ImmobaSil FS) in the bioreactors. Various factors influencing cell attachment, including serum concentration, cell to microcarrier ratio, agitation speed and agitation profile were investigated and the results were compared with those obtained using CultiSpher-G, and glass beads. It was found that the optimum serum concentration, cell-to- carrier ratio and agitation speed for the batch reactor with a working volume of 400 ml during the initial attachment period were 10% (vh), 12x103:l and 60 rpm, respectively.
The algorithm also allows the solution to be constrained as follows: 53 TABLE 1. Biochemical reactions in hybridoma cells AcCOA: Acetyl coenzyme A; DKG: a-ketoglutarate: ALA: Alanine; ARG: Arginine; ASN: Asparagine; ASP: Aspartate; BIOMAS: Biomass; CYS: Cysteine; C02: Carbon dioxide; FUM: Fumarate; GLC: Glucose; GLN: Glutamine; GLU: Glutamate; GLY: Glycine; HIS: Histidine; ILE: Isoleucine; LAC: Lactate; LEU: Leucine; LYS: Lysine; MAL: Malate; MET: Methionine; NH2: Ammonia; O2:Oxygen; OAA: Oxaloacetate; PHE: Phenylalanine; PRO: Proline; PYR: Pyruvate; SER: Serine; SUCCOA: Succinate coenzyme A; THR: Theonine; TYR: Tyrosine; VAL: Valine TABLE 2.
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